A rapid screening method for alkaline .BETA.-cyclodextrin glucanotransferase using phenolphthalein-methyl orange-containing-solid medium.
نویسندگان
چکیده
منابع مشابه
Deprotonation of beta-cyclodextrin in alkaline solutions.
Variable pH (13)C NMR and (1)H NMR spectroscopic studies of the beta-cyclodextrin (beta-CD) in alkaline aqueous solutions revealed that beta-CD does not deprotonate at pH<12.0. Further increase in solution pH results in the deprotonation of OH-groups adjacent to C-2 and C-3 carbon atoms of beta-CD glucopyranose units, whereas the deprotonation of OH-groups adjacent to C-6 carbon atoms is expres...
متن کاملMethyl orange as a screening reagent for organic bases.
and develop comparable regulatory programs for their state or (b) require intrastate clinical laboratories providing toxicological services to participate in the toxicology proficiency program now being separately developed by the National Center for Disease Control or the College of American Pathologists. The failure to provide adequate safeguards by mandatory drug-abuse proficiency testing op...
متن کاملInvestigation on Cyclodextrin Production with Cyclodextrin Glucanotransferase from Bacillus megaterium
The process of producing cyclodextrins from starch with cyclodextrin glucanotransferase from Bacillus megaterium has been studied. The effect of starch and enzyme concentrations was evaluated using optimal composite designs and the response surface methodology. Mathematical models describing the process in two overlapping areas of variation of the independent variables were developed. The model...
متن کاملScreening of mucoadhesive microparticles containing hydroxypropyl-beta-cyclodextrin for the nasal delivery of risperidone.
Interaction of the antipsychotic drug risperidone with hydroxypropyl-beta-cyclodextrin (HPBCD) in solution and in the solid state was studied with the aim of overcoming the limitations associated with nasal administration of low solubility drugs. Risperidone solubility studies revealed inclusion complex formation with a 1:1 stoichiometry. Low concentrations (0.1 w/v %) of hydroxypropylmethyl ce...
متن کاملGlucosylation of sucrose laurate with cyclodextrin glucanotransferase.
Sucrose monolauroyl esters were found to serve as substrates for cyclodextrin glucanotransferase (CGTase)-catalyzed transglucosidation reactions, affording new sucrose esters that have an additional 1-3 glucose residues on the pyranose ring of the sucrose moiety in the ester.
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1989
ISSN: 0002-1369,1881-1280
DOI: 10.1271/bbb1961.53.1167